Samsung said this week it has not considered acquiring RIM or licensing the BlackBerry maker's new mobile operating system.
A lack of support from potential partners such as Samsung could mean more trouble for RIM, which is seeking various options to turn around its embattled business.
Shares of RIM had risen more than 5 per cent early this week after an influential analyst said it may license the BlackBerry 10 system to Samsung.
"RIM has already lost its initiative in the smartphone market and what is left doesn't look really attractive to the likes of Samsung," said Lee Sei-cheol, an analyst at Meritz Securities.
"Should they have a deep patent pool, that might be the most appealing asset to potential acquirers."
Samsung, the world's largest smartphone maker, is the biggest seller of phones that run on Google's Android system, but it also makes phones using Microsoft's Windows as well as its proprietary software called bada.
Its strategy of making phones with multiple platforms had raised speculation it may also license BlackBerry's system to stretch its lead over rivals such as Apple and diversify away from Google, which now owns a handset manufacturing business after acquiring Motorola Mobility.
RIM plans to use its new operating system, known as BB10, in a next-generation line of BlackBerrys expected to launch early next year.
It is considered RIM's last hope of reversing BlackBerry's steady decline in market share.
RIM's shares have fallen more than 80 per cent since the beginning of 2011 when Apple and other smartphone makers started to widen their lead on RIM, which once dominated the business.